dlin mc3 dma Search Results


dlin mc3 dma  (Croda International Plc)
95
Croda International Plc dlin mc3 dma
Dlin Mc3 Dma, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pm41549419-47-8-25?v=Croda+International+Plc
Average 95 stars, based on 1 article reviews
dlin mc3 dma - by Bioz Stars, 2026-07
95/100 stars
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dlin-mc3-dma  (MedKoo Inc)
90
MedKoo Inc dlin-mc3-dma
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Dlin Mc3 Dma, supplied by MedKoo Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc07735425-119-0-4?v=MedKoo+Inc
Average 90 stars, based on 1 article reviews
dlin-mc3-dma - by Bioz Stars, 2026-07
90/100 stars
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ionizable lipids dlin-mc3-dma  (Cayman Chemical)
90
Cayman Chemical ionizable lipids dlin-mc3-dma
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Ionizable Lipids Dlin Mc3 Dma, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/bio_rxiv__2025__06__25__661517-131-8-11?v=Cayman+Chemical
Average 90 stars, based on 1 article reviews
ionizable lipids dlin-mc3-dma - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma lnps  (AstraZeneca ltd)
90
AstraZeneca ltd dlin-mc3-dma lnps
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Dlin Mc3 Dma Lnps, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pm39965058-132-11-2?v=AstraZeneca+ltd
Average 90 stars, based on 1 article reviews
dlin-mc3-dma lnps - by Bioz Stars, 2026-07
90/100 stars
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dlinmc3-dma lnps containing modified vegf-a mrna  (TriLink)
90
TriLink dlinmc3-dma lnps containing modified vegf-a mrna
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Dlinmc3 Dma Lnps Containing Modified Vegf A Mrna, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pm36806740-421-144-156?v=TriLink
Average 90 stars, based on 1 article reviews
dlinmc3-dma lnps containing modified vegf-a mrna - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma (mc3)  (NanoSoft LLC)
90
NanoSoft LLC dlin-mc3-dma (mc3)
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Dlin Mc3 Dma (Mc3), supplied by NanoSoft LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pm39087764-74-6-8?v=NanoSoft+LLC
Average 90 stars, based on 1 article reviews
dlin-mc3-dma (mc3) - by Bioz Stars, 2026-07
90/100 stars
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ionizable cationic lipid o -( z,z,z,z -heptatriaconta-6,9,26,26-tetraen-19-yl)-4-(n,n-dimethylamine) butanoate (mc3)  (Biofine International Inc)
90
Biofine International Inc ionizable cationic lipid o -( z,z,z,z -heptatriaconta-6,9,26,26-tetraen-19-yl)-4-(n,n-dimethylamine) butanoate (mc3)
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Ionizable Cationic Lipid O ( Z,Z,Z,Z Heptatriaconta 6,9,26,26 Tetraen 19 Yl) 4 (N,N Dimethylamine) Butanoate (Mc3), supplied by Biofine International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc05167785-208-6-13?v=Biofine+International+Inc
Average 90 stars, based on 1 article reviews
ionizable cationic lipid o -( z,z,z,z -heptatriaconta-6,9,26,26-tetraen-19-yl)-4-(n,n-dimethylamine) butanoate (mc3) - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma ((6z,9z,28z,31z)-heptatriacont-6,9,28,31-tetraene-19-yl 4-(dimethylamino)butanoate  (SAI Life Sciences Ltd)
90
SAI Life Sciences Ltd dlin-mc3-dma ((6z,9z,28z,31z)-heptatriacont-6,9,28,31-tetraene-19-yl 4-(dimethylamino)butanoate
a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of <t>DLin-MC3-DMA</t> SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.
Dlin Mc3 Dma ((6z,9z,28z,31z) Heptatriacont 6,9,28,31 Tetraene 19 Yl 4 (Dimethylamino)butanoate, supplied by SAI Life Sciences Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc09628342-46-0-6?v=SAI+Life+Sciences+Ltd
Average 90 stars, based on 1 article reviews
dlin-mc3-dma ((6z,9z,28z,31z)-heptatriacont-6,9,28,31-tetraene-19-yl 4-(dimethylamino)butanoate - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma  (AVT Co Ltd)
90
AVT Co Ltd dlin-mc3-dma
Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].
Dlin Mc3 Dma, supplied by AVT Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc09683711-109-11-12?v=AVT+Co+Ltd
Average 90 stars, based on 1 article reviews
dlin-mc3-dma - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma mc3  (ApexBio)
90
ApexBio dlin-mc3-dma mc3
Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].
Dlin Mc3 Dma Mc3, supplied by ApexBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pm35755271-128-0-7?v=ApexBio
Average 90 stars, based on 1 article reviews
dlin-mc3-dma mc3 - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma-lnp  (Genevant Sciences Inc)
90
Genevant Sciences Inc dlin-mc3-dma-lnp
Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].
Dlin Mc3 Dma Lnp, supplied by Genevant Sciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc08695324-46-0-6?v=Genevant+Sciences+Inc
Average 90 stars, based on 1 article reviews
dlin-mc3-dma-lnp - by Bioz Stars, 2026-07
90/100 stars
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dlin-mc3-dma  (Arbutus Biopharma)
90
Arbutus Biopharma dlin-mc3-dma
Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].
Dlin Mc3 Dma, supplied by Arbutus Biopharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dlin+mc3+dma/pmc08272596-226-14-19?v=Arbutus+Biopharma
Average 90 stars, based on 1 article reviews
dlin-mc3-dma - by Bioz Stars, 2026-07
90/100 stars
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Image Search Results


a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of DLin-MC3-DMA SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.

Journal: Nature nanotechnology

Article Title: Selective ORgan Targeting (SORT) nanoparticles for tissue specific mRNA delivery and CRISPR/Cas gene editing

doi: 10.1038/s41565-020-0669-6

Figure Lengend Snippet: a , Addition of a supplemental component (termed a SORT molecule) to traditional LNPs systematically alters the in vivo delivery profile and mediates tissue specific delivery as a function of the percentage and biophysical property of the SORT molecule. This methodology successfully redirected multiple classes of nanoparticles. b , 5A2-SC8 SORT LNPs were formulated as indicated to make series of LNPs with 0% to 100% SORT lipid (fraction of total lipids). Here, inclusion of a permanently cationic lipid (DOTAP) systematically shifted luciferase protein expression from the liver to spleen to lung as a function of DOTAP percentage. c , Quantification data demonstrated that SORT molecule percentage is the most important factor for tissue-specific delivery. Data were shown as mean±s.e.m. (n=4 biologically independent animals). d , Relative luciferase expression in each organ demonstrated that fractional expression could be predictable tuned (0.1 mg/kg Luc mRNA, IV, 6h). Data are shown as mean±s.e.m. (n=4 biologically independent animals). e , Inclusion of an anionic SORT molecule enabled selective mRNA delivery to the spleen. Luciferase expression was observed only in spleen when introducing 18PA lipid into mDLNPs up to 40%. f , Ex vivo images of luminescence in major organs of DLin-MC3-DMA SORT LNPs and C12–200 SORT LNPs (0.1 Luc mRNA mg/kg, IV, 6h). g , Details of selected SORT molecule formulations.

Article Snippet: DLin-MC3-DMA was purchased from MedKoo Biosciences.

Techniques: In Vivo, Luciferase, Expressing, Ex Vivo

Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].

Journal: Science Advances

Article Title: Structural and biochemical characteristics of mRNA nanoparticles determine anti–SARS-CoV-2 humoral and cellular immune responses

doi: 10.1126/sciadv.abo1827

Figure Lengend Snippet: Formulations of LNP, CNE, and Lipo. DMG-PEG 2000 , 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000; DOPE, 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine; DOTAP, dioleylhydroxypropyl-3- N , N , N -trimethylammonium; DSPC, 1,2-dioctadecanoyl- sn -glycero-3-phophocholine; DSPE-PEG 2000 , distearoyl- sn -glycero-3-phosphoethanolamine- N -[maleimide (polyethylene glycol)-2000].

Article Snippet: mRNA@LNP was synthesized by mixing one volume of lipid mixture of DLin-MC3-DMA (AVT Co. Ltd., Shanghai, China), 1,2-dioctadecanoyl- sn -glycero-3-phophocholine (AVT Co. Ltd., Shanghai, China), cholesterol, and 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (AVT Co. Ltd., Shanghai, China) (50:10:38.5:1.5, molar ratio) in ethanol and three volumes of mRNA [1:25 (w/w) mRNA to lipid, nitrogen/phosphate (N/P) ratio = 6] in citrate buffer (pH 4.0; 10 mM).

Techniques: